Photoreaction based modification and protein patterning on cyclic olefin copolymer surfaces with a nonbiofouling polymer

초록

Chemical functionalization on solid surface is a demand for the investigation on interfacial reaction-phenomena. In the case of metal surfaces, self-assembled monolayers (SAMs) have been intensively used for surface modification. However, a limited number of solid substrates are suitable for SAMs because covalent bonds between a surface and a coating molecule are required. Further, chemical functionalization of some polymeric surfaces, such as polycarbonate and cyclic olefin copolymer (COC), is tough due to the lack of chemically reactive groups. COC, the copolymer of 2-norbonene and ethylene, has desirable properties for microfluidics, biochips, and sensor devices, since COC shows characteristics of high transparency, low autofluorescence, birefringence, robustness, low water uptake, and resistance to organic solvents. To functionalize chemically inert COC surfaces with a minimizing biofouling, we have developed a photoreaction-based method for forming functionalizable and nonbiofouling poly(oligo(ethylene glycol) methacrylate (pOEGMA) film on COC. Perfluoroaryl azide was employed for chemical functionalization of the COC surface with a post-functionalizable pOEGMA. Further, biotin was linked to the pOEGMA layer, and a protein (streptavidin) micropattern was generated using microcontact printing to confirm the spatioselective introduction of chemical functional groups on the pOEGMA-coated COC surface and the nonbiofouling features of the pOEGMA/COC film.