Chemical Functionalization of a Polymer Surface and Its Biological Application

초록

Immobilization of biomolecules onto polymer surfaces has recently exploited in the areas of biosensors, medical devices, and microfluidic devices due to the favorable properties of polymer materials such as mechanical flexibility, biocompatibility, low density, and low cost. Various polymers including polycarbonate, poly(methyl methacrylate), and cyclic olefin copolymer (COC) have been studied for the fabrication of micrometer-scaled devices. Specifically, COC, a poly(ethylene-co-norbornene) derivative, has emerged as a suitable substrate for microfluidic devices and biochips, because the high UV transparency of COC enables photolithographic fabrication under on-chip conditions. To functionalize chemically inert COC surfaces with a minimizing biofouling, we have studied a photoreaction-based method for forming functionalizable and nonbiofouling poly(oligo(ethylene glycol) methacrylate (pOEGMA) film on COC. Further, we demonstrated an estimation of a surface coverage of the antibody immobilized onto the surface with the immuno-gold nanoparticles (Au NP). The processes were confirmed by water contact angle measurement, FT-IR spectroscopy, and FE-SEM. The strategy demonstrated in this work could be applied to functionalizations of other polymeric materials and determination of the binding capacity of analytes in biosensors and microfluidic devices.