Polymer-based signal amplification of eosin-functionalized macrophotoinitiators for colorimetric detection

초록

Polymerization-based amplification (PBA) has recently studied as a promising signal amplification technique for use in molecular diagnostic devices. This technic couples polymerization reactions to biological binding events, and cross-linked hydrogel polymers serve as an amplified readout to report on the number of binding events without the requirement of expensive instrumentation. In this work, the eosin-tertiary amine co-initiation system, activated by visible light, has proven utility in this context when an average of three eosin molecules are coupled to a protein detection reagent. To evaluate the capacity of the macrophotoinitiators to detect molecular recognition, we coupled neutravidin to these molecules to recognize biotin-labeled DNA immobilized on biochip test surfaces. In eosin macroinitiators system, a non-monotonic relationship was observed between the number of initiators per binding event and the observed detection sensitivity.