Colorimetric Detection Using Polymerization-Based Signal Amplification with Dye-Based Macroinitiators

초록

Coupling polymerization initiators to molecular recognition events provide the ability to amplify these events and detect them using the formation of a cross-linked polymer as an inexpensive readout that is visible to the naked eye. The fluorescein or eosin/ tertiary amine co-initiation system, activated by visible light, has proven utility in this context when a number of dye molecules are coupled to a protein detection reagent. The present work addresses the question of how detection sensitivity is impacted when the number of dye molecules per binding event increases.