Tuning lateral packing densities of pOEGMA on gold surfaces and its impact on the amount of immobilized streptavidin

초록

Biofouling, the accumulation of biological matters on a surface causing the deterioration of the device, is a problematic issue in a wide range of fields, such as biomedical devices/implants, biosensors, drug delivery carriers, and food industry. Recently, specific immobilization of biomolecules on non-biofouling polymeric films has received much attention, because it can serve as a platform for achieving highly sensitive biochips and biomedical devices. Previously reported results not only indicated that the amount of immobilized protein can be controlled by selection of reactive sites, but also encourage us to understand binding behaviors. Thus, we investigated binding behaviors of streptavidin with the different lateral packing densities of biotin-functionalized, non-biofouling pOEGMA brushes. pOEGMA was synthesized by surface-initiated polymerization from the mixed SAMs with different mole fractions of initiators on gold surfaces.