Reversible polymerization from surface-anchored DNA by enzymatic processes

초록

Reversible polymerization has emerged as a new strategy in polymer synthesis, where synthetic polymers and materials possess the covalent bonds that are capable of reversible connection and disconnection by external stimuli, such as heat or light. We designed a system of DNA-based reversible polymerization on gold surfaces with several different self-priming DNA oligomers and restriction enzymes. Also, we investigated the surface-initiated DNA polymerization, de-polymerization, and re-polymerization. Since the self-priming oligonucleotides were polymerized by the process of sequence-specific recognition, the formed DNA polymer films were selectively de-polymerized by the right restriction enzyme and re-polymerized from remaining self-priming oligonucleotides on the surfaces.